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A target expression threshold dictates invader defense and prevents autoimmunity by CRISPR-Cas13. Vialetto E, Yu Y, Collins SP, Wandera KG, Barquist L, Beisel CL. Cell Host Microbe. 2022 Aug 10;30(8):1151-1162.e6. doi: 10.1016/j.chom.2022.05.013. Epub 2022 Jun 10.
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Anti-CRISPR prediction using deep learning reveals an inhibitor of Cas13b nucleases. Wandera KG, Alkhnbashi OS, Bassett HVI, Mitrofanov A, Hauns S, Migur A, Backofen R, Beisel CL. Mol Cell. 2022 Jul 21;82(14):2714-2726.e4. doi: 10.1016/j.molcel.2022.05.003. Epub 2022 May 31.
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Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems. Wandera KG, Beisel CL. Methods Mol Biol. 2022;2404:135-153. doi: 10.1007/978-1-0716-1851-6_7.
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An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system. Wandera KG, Collins SP, Wimmer F, Marshall R, Noireaux V, Beisel CL. Methods. 2020 Feb 1;172:42-50. doi: 10.1016/j.ymeth.2019.05.014. Epub 2019 May 21.
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| Last update Publications 18.06.2022 |
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